24-05-2019, 04:31 PM
Does anyone actually know? I came across this paper, but I only recognized progesterone.
https://academic.oup.com/endo/article-ab...34/2775093
Only the abstract is available
Abstract
Ovaries from non-pregnant cows and heifers, obtained shortly after slaughter, were extracted with ethyl acetate and then defatted in cold 70% methanol.
The resulting extracts were chromatographed on paper and the isolated compounds identified by: (1) mobility on paper, (2) formation of derivatives and (3) ultra-violet and sulfuric acid spectra.
Quantitative estimates from ultra-violet spectra indicated 4.33 μg. of progesterone and 0.79 μg- of Δ4-pregnene-20β-ol-3-one per gram of ovarian tissue. A trace of an ultra-violet absorbing compound that chromatographed like Δ4-androstene-3,17-dione or Δ4-pregnene-20α-ol-3-one and a very polar material were also detected.
https://academic.oup.com/endo/article-ab...34/2775093
Only the abstract is available
Abstract
Ovaries from non-pregnant cows and heifers, obtained shortly after slaughter, were extracted with ethyl acetate and then defatted in cold 70% methanol.
The resulting extracts were chromatographed on paper and the isolated compounds identified by: (1) mobility on paper, (2) formation of derivatives and (3) ultra-violet and sulfuric acid spectra.
Quantitative estimates from ultra-violet spectra indicated 4.33 μg. of progesterone and 0.79 μg- of Δ4-pregnene-20β-ol-3-one per gram of ovarian tissue. A trace of an ultra-violet absorbing compound that chromatographed like Δ4-androstene-3,17-dione or Δ4-pregnene-20α-ol-3-one and a very polar material were also detected.